Fig. 1: Analysis of epidermal cells in non-dividing cell files of Arabidopsis hypocotyls.

a, Heatmap quantification of cell length in the non-dividing cell files of Col-0, pif4-2, cdfq and pif4-2 cdfq grown under SDs for 7 days. Scale bar, 500 µm. Caption image shows representative transversal section of Col-0 hypocotyl. L, large non-dividing cell file; S, small dividing cell file. b,c,d, The mean cell number (b), cell length (c) and cell width (d) of the non-dividing cell files of hypocotyls. Box plots in panels c and d show the minimum, 25th percentile, median, 75th percentile and maximum of data points. Different letters in panels b, c and d represent significant differences among genotypes (P < 0.05), using ANOVA followed by Tukey’s pairwise multiple comparison, P = 0.325, 7.99 × 10−7 and 9.64 × 10−5 in b, c and d, respectively); n = 8 cell files examined over 4 hypocotyls, cell numbers are presented in Fig. 1b.