Extended Data Fig. 8: Gel-shift analysis of the interactions between PIF4bHLH WT protein and different length of DNA probes.

Gel-shift analysis of the interactions between PIF4bHLH WT protein and different length of DNA probes (as described in Fig. 4f). Several different sizes of complexes of PIF4bHLH bound to the longer DNA fragment, ‘b’ could be reduced using shorter DNA fragments (fragment ‘c’) containing only one G-box. EMSA assays were performed twice with similar results.