Extended Data Fig. 2: Flowering time and hypocotyl length of transgenic plants carrying CDF2::HA-CDF2.
Flowering time and hypocotyl length of transgenic plants carrying CDF2::HA-CDF2. a, Flowering time of WT, cdf2-1 and CDF2::HA-CDF2 transgenic lines in long-day conditions. The total leaf number was counted for plants of WT, cdf2-1, and T3 homozygous populations of three independent transgenic lines. Data are presented as means ± SEM; n = 14 for WT, cdf2-1, #7 and #3 and n = 13 for #10 in a. Box plots in panel a show the minimum, 25th percentile, median, 75th percentile and maximum of data points. n = (14, 14, 14, 14, 14). Letters in panels a show significant differences between genotypes (P < 0.05, using one-way ANOVA followed by Tukey test, two sided), P = 7.254*10−45. b, Hypocotyl length of 9-day-old seedlings of WT, cdf2-1, CDF2::HA-CDF2 (#3), cdf2-1 and CDF2::HA-CDF2 (#3) cdf2-1 pif4-2 in short-day conditions. Box plots in panel b show the minimum, 25th percentile, median, 75th percentile and maximum of data points. n = (30, 29, 29, 30). Letters in panels b show significant differences between genotypes (P < 0.05, using one-way ANOVA followed by Tukey test, two sided), P = 1.298*10−42. c, RT-qPCR analysis of HA-CDF2 mRNA levels in cotyledons and hypocotyls of cdf2-1 or cdf2-1 pif4-2 mutants. All values are normalized to APA1 levels. Data are presented as mean values ± SEM of three biological replicates. Statistical significance was determined by pairwise one-sided t-test (P = 0.4964 and 0.3869 in cotyledon and hypocotyl, respectively). Significant P < 0.05. NS, not significant. d, Western blotting analysis of the abundance of HA-CDF2 protein in cotyledons and hypocotyls in cdf2-1 or cdf2-1 pif4-2 mutants. Actin was used as the loading control. Western blots represent one of three independent biological replicates. Tissues were harvested at ZT-0.5 to ZT-1 from 7-day-old SD-grown seedlings in c and d.
