Fig. 2: Pharmacological and genetic perturbation of MT guidance of CSCs disrupts their organization at indentations.
a, Projections of MTs/tubulin (magenta), CESA3 (green) and merged channels in single cotyledon cells (yellow outlines) at 48 hpd after 4 h DMSO (top) and oryzalin (ORYZ) treatment (bottom). Scale bar, 10 µm. b, Zoom-ins of regions marked in a showing more organized CESA3 trajectories in DMSO- as compared with ORYZ-treated cells. Scale bar, 5 µm. c,d, Local CESA3 anisotropy (c) within a 1.8 µm margin from the cell contour, and CESA3-curvature correlation coefficients (d) in DMSO- and ORYZ-treated cells. In brackets: number of cells. Means ± 95% confidence intervals; Welch’s unpaired t-test (two-tailed, P values). e, Projections of MTs (magenta), CESA3 (green) and merged channels in single cotyledon cells (yellow outlines) at 48 hpd for wild-type (top) and pom2-8 mutant cells (bottom). Scale bar, 10 µm. f, Zoom-ins of regions highlighted in e showing more organized CESA3 trajectories in wild type as compared with pom2-8. Scale bar, 5 µm. g, Co-localization of CESA3 and MTs as measured by Pearson’s correlation coefficients in wild type and pom2-8. In brackets: number of cells. Means ± 95% confidence intervals; Welch’s unpaired t-test (two-tailed, P values). h,i, Local CESA3 anisotropy (h) and CESA3-curvature correlation coefficients (i) in wild type and pom2-8. In brackets: number of cells. Means ± 95% confidence intervals; Welch’s unpaired t-test (two-tailed, P values). See statistics and reproducibility in Methods.
