Extended Data Fig. 8: Fig. 8. Protein sequence alignment and phylogenetic analysis of SPWR1.
From: Natural allelic variation confers high resistance to sweet potato weevils in sweet potato
a, Protein sequence alignment of SPWR1 in G87, N73, and N28. Blue box indicates the conserved WRKY domain. Red boxes and phrases indicate the amino acid substitutions and indel site. Sequences were aligned with Clustal X. b, Phylogenetic tree shows the predicted SPWR1 homologs in different plant species. NCBI accessions: SPWR1 (Ipomoea batatas), OM283291; WRKY-like (Ipomoea triloba), XP_031129806.1; WRKY-like (Ipomoea nil), XP_019170409.1; WRKY-like (Nicotiana attenuata), XP_019233122.1; WRKY-like (Nicotiana tabacum), XP_016453841.1; WRKY-like (Solanum tuberosum), XP_006352253.1; WRKY-like (Salvia splendens), XP_042022560.1; WRKY-like (Sesamum indicum), XP_020551475.1; WRKY-like (Olea europaea var. sylvestris), XP_022886946.1; WRKY-like (Actinidia rufa), GFZ16403.1; WRKY-like (Theobroma cacao), XP_007040478.2. Phylogenetic analysis was performed by maximum likelihood (ML) method using MEGA7.1 with 1000 bootstrap replicates to build the ML tree.
