Extended Data Fig. 9: Protein and promoter sequence alignment and phylogenetic analysis of SPWR2.
From: Natural allelic variation confers high resistance to sweet potato weevils in sweet potato
a, Protein sequence alignment of SPWR1 in G87, N73, and N28. Blue box indicates the conserved DHQS domain. Red boxes and phrases indicate the amino acid substitutions. b, Promoter sequence alignment of SPWR2 in G87, N73, and N28. Red boxes and phrases indicate the Indels. Blue box indicates the W-box element in Indel1. Sequences were aligned with Clustal X. c, Phylogenetic tree shows the predicted SPWR2 homologs in different species. NCBI accessions: SPWR2 (Ipomoea batatas), OM283290; DHQS-like (Ipomoea nil), XP_031109196.1; DHQS-like (Nicotiana tabacum), XP_016464940.1; DHQS-like (Solanum tuberosum), XP_006340763.1; DHQS-like (Solanum lycopersicum), NP_001233863.1; DHQS-like (Olea europaea var. sylvestris), XP_022885746.1; DHQS-like (Coffea arabica), XP_027073560.1; DHQS-like (Arabidopsis thaliana), NP_001030791.1; DHQS-like (Oryza sativa), XP_015612522.1; DHQS-like /aroB (Escherichia coli), NP_417848.1. Phylogenetic analysis was performed by maximum likelihood (ML) method using MEGA7.1 with 1000 bootstrap replicates to build the ML tree.
