Extended Data Fig. 10: Generation and expression identification of the SPWR1 and SPWR2 transgenic sweet potatoes.
From: Natural allelic variation confers high resistance to sweet potato weevils in sweet potato
a, Southern blot analysis of the transgenic sweet potato lines using a DIG-labeled probe for detection of HPT (Hygromycin Phosphotransferase) gene. M, DIG-labeled lambda/Hind III Marker (TIANDZ, Inc., China); W, the wild type G87; lanes 1-4, OE-SPWR1 transgenic lines; lanes 5-8, OE-SPWR2 transgenic lines; 9-10, SPWR1-RNAi transgenic lines; lanes 11-12, SPWR2-RNAi transgenic lines. All genomic DNA was digested with Hind III. b,c, Gene expression analysis of SPWR1 (b) and SPWR2 (c) in different transgenic lines. d, The phenotype of SPWR1 and SPWR2 transgenic lines at 3 WAC. Scale bar = 1 cm. Data are presented as means ± SD of nine biological replicates. Asterisks indicate significant difference (Two-tailed Student’s t-test, ** P < 0.01, *** P < 0.001). Tukey’s multiple comparison was performed by One-way ANOVA, and statistically significant differences are indicated by different lower-case letters. All exact P values were shown in the relative Source Data Extended Data Fig. 10.
