Extended Data Fig. 2: TALED of chloroplast target constructs assembly schematic.
From: Targeted A-to-G base editing of chloroplast DNA in plants
Each TALED pairs were cloned into a different T-vector: Left TALE(L-1397N) cloned into RPS5Apro-CTS-35Ster-Aat II-Pme I T-vector and Right TALE (R-1397C-AD) cloned into Aat II-RPS5Apro- CTS-35Ster-PmeI. After digesting each vector with Aat II and Pme I, Aat II-RPS5Apro-CTS-Right TALE-1397C-AD-35Ster-PmeI was ligated to a vector with pRPS5Apro-CTS-Left TALE-1397N-35Ster-Aat II-Pme I, and the TALED pair was cloned into one vector. 1397 N and 1397C-ABE8.0 (ABE8e), which intersected in Left TALE and Right TALE, were also constructed.
