Extended Data Fig. 4: RIH validation of marker genes used for cell type annotation. | Nature Plants

Extended Data Fig. 4: RIH validation of marker genes used for cell type annotation.

From: Single-cell RNA sequencing provides a high-resolution roadmap for understanding the multicellular compartmentation of specialized metabolism

Extended Data Fig. 4

Paraffin-embedded serial cross-sections from 1.8–2.0 cm leaves were hybridized with digoxigenin-labeled transcripts. Sections were hybridized with sense and antisense RNA probes for NLTP2, SABP2, CB21 and PRS2 to localize their mRNAs in C. roseus leaves. The identified cell types are indicated by yellow arrows: CB21, mesophyll cell; SABP2, internal phloem-associated parenchyma cell; NLTP2, epidermal cell; PRS2, vascular cell. le, lower epidermis; ue, upper epidermis; pm, palisade mesophyll cells; and sm, spongy mesophyll cells.

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