Extended Data Fig. 9: Protein and transcript levels of phosphonull and phosphomimetics PUB30/31 in Arabidopsis seedlings.
From: Direct attenuation of Arabidopsis ERECTA signalling by a pair of U-box E3 ligases
(a) The protein levels of wild-type or various phosphosite mutated PUB30 in the Arabidopsis complementation lines. Total proteins were isolated from 7-d-old seedlings and probed by anti-FLAG antibody. Anti-actin antibody was used as a native control. (b) The protein levels of wild-type or various phosphosite mutated PUB31 in the Arabidopsis complementation lines. Total proteins were isolated from 7-d-old seedlings and probed by anti-FLAG antibody. Anti-actin antibody was used as a native control. (c) The transcript levels of PUB30 by RT-qPCR analysis in pub30 pub31 and wild type or various phosphosite mutated PUB30 complementation lines. Error bars represent SD (n = 3). (d) The transcript levels of PUB30 by RT-qPCR analysis of PUB31 in pub30 pub31 and wild type or various phosphosite mutated PUB30 complementation lines. Error bars represent SD (n = 3).
