Extended Data Fig. 5: Phylogenetic tree of IIIb bHLH TFs.

A maximum-likelihood bHLH phylogenetic tree of subfamilies IIIb, III (a + c) (light blue), and III(d + e) (grey, outgroup) is shown. Numbers at branches indicate bootstrap values calculated from 1,000 replicates. The scale bar indicates the substitution rate per residue. IIIb bHLHs are divided into 2 groups: ICE/SCRM clade (orange) and NFL clade (magenta). Species are abbreviated as follows: Mp, M. polymorpha (liverwort); Lc, L. cruciata (liverwort); Pp, P. patens (moss); Cepur, Ceratodon purpureus (moss); Aagr, Anthoceros agrestis (hornwort); Sm, Selaginella moellendorffii (lycophyte); AmTr, Amborella trichopoda (basal angiosperm); Os, Oryza sativa (monocot); At, A. thaliana (dicot). Arrows indicate MpICE1 (Mp4g04910) and MpICE2 (Mp4g04920). For the phylogenetic construction of subfamilies III(a + c) and III(d + e), we used amino acid sequences from only A. thaliana and M. polymorpha.