Extended Data Fig. 4: Generation and phenotypes of MpSETA knock-out lines.

a, Structure of the MpSETA locus disrupted by homologous recombination. Knock-out lines have a deletion in the bHLH domain coding region. White boxes indicate the exons of the MpSETA coding sequence. DT-A, diphtheria toxin A fragment gene; Hgr^R, hygromycin- resistance gene. b, Genotyping of the Mpseta^ko lines used in this study to distinguish sex. rbm27, a male-specific marker; rhf73, a female-specific marker. c, Genotyping of the Mpseta^ko lines. The position of the primers used for PCR is shown in (a). M, Male; F, Female. d, RT-PCR to confirm the loss of the full-length MpSETA transcript in Mpseta^ko lines in 21 DPF sporophytes. MpEF1α was used as an internal control. e, Spermatogenesis process in the wild type (WT) and Mpseta^ko lines. All the images are at the same scale. The experiments in this figure were repeated at least three times with similar results. Bars, 10 μm (e).