Extended Data Fig. 6: UMP1R2115 enhances defence responses against M. oryzae, which is inhibited by MG132.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a and b, Expression analysis of defence-related genes in leaves of TP309, UMP1R2115 (a), and mutant ko-11 (b) lines inoculated with M. oryzae strain GZ8. OsUbi was used as the reference gene. The expression of indicated genes in TP309-0 hpi (hours post inoculation) was set as the control. Data are mean ± s.d. (n = 3 biological replicates). P values were determined by two-sided unpaired t-test, compared to TP309 at corresponding time points. c and i, RT-qPCR analysis of OsUMP1 in leaves of R2115 (c) and LTH (i) inoculated with GZ8. OsUbi was used as the reference gene. d and j, Measurements of 26S proteasome amount in R2115 (d) and LTH (j). e and k, Measurements of 26S proteasome activity in R2115 (e) and LTH (k). f and l, Measurements of POD amount in R2115 (f) and LTH (l). g and m, Measurements of CAT amount in R2115 (g) and LTH (m). h and n, Quantification of H2O2 in R2115 (h) or LTH (n). Data are mean ± s.d. (n = 3 biological replicates for c–n). o, Measurements of 26S proteasome activity in leaves of UMP1R2115 transgenic lines infected with M. oryzae strain GZ8 at 5 dpi. MG132 treatment was conducted at 1 day before M. oryzae inoculation. p and q, Measurements of the abundance of POD (p) and CAT (q). r, Quantification of H2O2. Data are mean ± s.d. (n = 3 biological replicates for o–r). P values were determined by two-sided unpaired t-test, compared to corresponding water-treated controls (o–r).
