Extended Data Fig. 7: Rice E3 ligase APIP6 promotes the degradation of OsCatB but not OsAPX8.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a, Sequence alignment of three rice catalases OsCatA, OsCatB, and OsCatC using DNAMAN software (version 5.2.2). Black shading sites indicate 100% homology, while turquoise shading sites indicate ≥50% homology. Note that the overall homology between OsCatB and OsCatC is 83.9%. b, Yeast-two-hybrid assay for detecting interaction of APIP6 with OsCatB/OsAPX8. DDO, SD/-Leu-Trp. QDO, SD/-Leu-Trp-His-Ade. c and d, Protein degradation assay in rice protoplasts. OsCatB-HA (c) or OsAPX8-HA (d) were co-expressed with APIP6-GFP or APIP6H58Y-GFP in rice protoplasts. The proteasome inhibitor MG132 (50 μM) was added at 12 h prior to sampling. LUC-Myc was used as an internal control.
