Extended Data Fig. 8: Overexpressing OsAPX8 or OsCatB in UMP1R2115 plants and expression analysis of defence-related genes in UMP1R2115 and TP309 plants treated with chitin or flg22.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a and b, Expression level of OsAPX8 (a) or OsCatB (b) in UMP1R2115 plants overexpressing OsAPX8 or OsCatB. The expression level was determined by RT-qPCR using OsUbi as the reference gene and null segregant as the control. Values are mean ± s.d. (n = 3 biological replicates). P values were determined by two-sided unpaired t-test, compared to the null segregant. c and d, Expression analysis of defence-related genes in TP309 and UMP1R2115-1 plants treated with chitin (40 μg ml−1; c) or flg22 (20 nM; d). OsUbi was used as the reference gene. TP309-0 hpt (hours post treatment) was set as the control. Values are mean ± s.d. (n = 3 biological replicates).
