Extended Data Fig. 3: The UMP1R2115 allele confers non-race-specific broad-spectrum resistance to M. oryzae.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a, Expression level of OsUMP1 in OsUMP1R2115 transgenic plants (T0 generation). The expression level of OsUMP1 was determined by RT-qPCR using OsUbi as the reference gene and TP309 as the control. Values are mean ± s.d. (n = 3 biological replicates). P values were determined by two-sided unpaired t-test, compared to TP309. b, Disease phenotype of indicated rice lines (T0 generation) at 7 days post inoculation (dpi) with M. oryzae GZ8. c and d, Disease phenotype and OsUMP1 expression of UMP1R2115-1 and UMP1R2115-13 segregants infected with M. oryzae GZ8. RT-qPCR analysis was performed with OsUbi as the reference gene and TP309 as the control. Values are mean ± s.d. (n = 3 biological replicates). e, Disease phenotype of TP309 and UMP1R2115 lines inoculated with a panel of M. oryzae strains. f, Quantification of lesion length in (e). Data are mean ± s.d. (n = 6 biological replicates). Significant difference was determined by two-sided unpaired ttest (* P < 0.05, ** P < 0.01).
