Extended Data Fig. 4: OsUMP1 partially rescues heat sensitivity of S. cerevisiae ump1 mutant and is required for tolerance to canavanine.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a, Protein sequences of UMP1 homologs in different plant species were downloaded from the Uniprot database (https://www.uniprot.org), and aligned using DNAMAN software (version 5.2.2). Black, magenta, and turquoise shading sites indicate homology of 100%, ≥75%, and ≥50%, respectively. b, Phylogenetic tree was generated with MEGA5.1 using the Muscle method for sequence alignment and the Neighbor-Joining method for tree construction. The accession numbers for UMP1 homologs are as below: Oryza punctata (A0A0E0KFV5), Oryza meridionalis (A0A0E0D398), Oryza glumipatula (A0A0D9YZR5), Leersia perrieri (A0A0D9VM90), Hordeum vulgare (F2CQM0), Triticum aestivum (A0A3B6QNA6), Brachypodium distachyon (I1IDJ7), Panicum hallii (A0A2S3GU64), Sorghum bicolor (A0A194YTW9), Zea mays (B4FLC7), Ananas comosus (A0A199VSL7), Arabidopsis thaliana (Q94B05), Punica granatum (A0A218XIA6) and Cucumis sativus (A0A0A0KUK6). UMP1 homologs in Homo sapiens (Q9Y244), Mus musculus (Q9CQT5) and Saccharomyces cerevisiae (P38293) were included as outgroups for phylogenetic analysis. c, Yeast cells of wild-type (WT), Scump1 mutant, and Scump1 transformed with rice UMP1R2115 were incubated at 28 °C or 37 °C for 48 h. d, Seedlings of TP309, ko-11, and UMP1R2115 lines were grown in 50 μM canavanine for 14 days. e, Changes of shoot and root length of TP309, ko-11, and UMP1R2115 seedlings under 50 μM canavanine treatment. Values are mean ± s.d. The numbers of biological replicates (n) are indicated in the figure.
