Extended Data Fig. 5: OsUMP1 regulates proteasome function and affects protein ubiquitination and accumulation in rice.
From: A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens
a and b, Relative proteasome activity normalized to proteasome amount (related to Fig. 2b,d) in leaves of UMP1R2115 (a) and ko-11 (b) lines upon infection with M. oryzae GZ8. Values are mean ± s.d. (n = 3 biological replicates). P values were determined by two-sided unpaired t-test, compared to TP309 at 0 hpi. c, Detection of ubiquitination of total proteins in TP309, UMP1R2115, and ko-11 plants. Seedlings of indicated lines were treated with/without MG132 (50 μM) for 12 h prior to M. oryzae infection. Samples were collected at 12 h post M. oryzae infection for western blot analysis with a ubiquitin antibody. Detection with a HSP antibody was used for loading normalization. d and e, GO enrichment analysis of differentially accumulated proteins (DAPs) between TP309 and UMP1R2115-1. Leaves from four-leaf-stage seedlings were used for iTRAQ proteomic analysis. Top ten enriched GO terms were presented for up-regulated (d) or down-regulated (e) DAPs. Rich factor refers to the ratio of DAP number against annotated protein number in background genome for a certain term. Term of interest was indicated by a red rectangle. Significance was determined by Fisher’s exact test (P < 0.05).
