Extended Data Fig. 10: Vesicular trafficking pathways affected in the p34 mutants.
a, secRFP localization in roots of 5-day-old Col-0 and p34iCRISPR-1 plants grown on DMSO and 10 μM β-oestradiol (Est). b, Immunofluorescence staining of KNOLLE in the root meristem of Col-0, p34iCRISPR-1 and p34iCRISPR-2 plants treated with 10 μM Est. c, PIN2-GFP localization in the roots of 5-day-old Col-0 and p34iCRISPR-1 plants grown on DMSO and 10 μM Est in the dark. d, Percentage of epidermal cells with GFP signals in the vacuoles as shown in (c), Error bars represent SD. e, Morphology of the lytic vacuoles in root cells of 7-day-old Col-0, p34iCRISPR-1 and p34iCRISPR-2 plants grown on DMSO and 10 μM Est. Images were taken after 2 h of staining with 4 μM FM4-64. Scale bars, 10 µm (a-c,e), The imaging of all genotypes was repeated three times. One representative experiment is shown.
