Extended Data Fig. 6: pRBE LUBS is required for LFY-UFO-dependent activation.
a, IGB view of pRBE showing LFY ChIP-seq in inflorescences (light blue)25 or seedlings (dark blue)26, LFY-UFO ampDAP-seq (yellow), LFY ampDAP-seq (pink)48, numbers indicate read number range (top). Identification of LUBS in pRBE (bottom). Predicted binding sites using dLUBS and mLUBS models from Fig. 2e and LFY PWM with dependencies68, y-axis represents score values (bottom). The best binding sites correspond to the less negative score values. Studied LUBS is indicated with a purple square. b, EMSA with probes corresponding to pRBE LUBS, WT or with URM mutated. c, pRBE activation in Arabidopsis protoplasts. Effect of mutations (underlined) in URM (red) and in LFYBS (blue) bases of pRBE LUBS were assayed. Data represent averages of independent biological replicates and are presented as mean ± SD, each dot representing one biological replicate (n = 4). One-way ANOVA with Tukey’s multiple comparisons test. One-way ANOVA were performed with data from the same effector, and stars represent a statistical difference compared to WT promoters (****: p < 0.0001). d, In vivo analysis of pRBE::GUS fusions. The percentage of transgenic lines with RBE pattern, unusual pattern or absence of staining was scored (top; χ² test, **: p < 0.01). n = number of independent lines. Unusual pattern refers to staining in unexpected tissues, each pattern seen in a single line. Representative pictures of plants with no staining (bottom left) and a RBE pattern (bottom right) are shown (scale bar, 50 µm). e, In vivo analysis of pRBE::GUS fusions. Same as in (d), with another view showing staining in the four petal primordia (scale bar, 50 µm). Source data are available in Supplementary Data 4.
