Extended Data Fig. 9: UFO binds DNA and LFY DBD. | Nature Plants

Extended Data Fig. 9: UFO binds DNA and LFY DBD.

From: The F-box protein UFO controls flower development by redirecting the master transcription factor LEAFY to new cis-elements

Extended Data Fig. 9

a, A representative micrograph of the ASK1-UFO-LFY-DNA complex in vitreous ice (scale bar, 20 nm). b, Selected 2D class averages of the particles submitted to ab initio reconstruction and heterogeneous refinement for 3D classification. c, Intermediate reconstructions of the 3D classes after heterogeneous refinement. d, Final reconstructions of ASK1-UFO-LFY-DNA complexes (involving either a LFY-DBD monomer (pink) or a LFY-DBD dimer (gray)) after Non-Uniform refinement. e, Unprocessed AlphaFold2 model for ASK1 (top, purple; uniprot ID, Q39255), UFO (middle, red; uniprot ID, Q39090) and the LFY-DBD dimer/DNA crystallographic structure (bottom, pale and dark blue for the LFY-DBD dimer and green for the DNA; PDB, 2VY1). f, Cryo-EM density map color-coded by fitted molecule. Note the kink on DNA induced by the presence of UFO. g, Heat map of the angular distribution of particle projections contributing for the final reconstruction of the complete ASK1-UFO-LFY-DNA complex (with a LFY-DBD dimer). h, Gold-standard Fourier shell correlation (FSC) curves. The dotted line represents the 0.143 FSC threshold, which indicates a nominal resolution of 6.4 Å for the unmasked (red) and 4.3 Å for the masked (blue) reconstruction. i, View of the post-processed map of the complete ASK1-UFO-LFY-DNA complex, colored according to the local resolution.

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