Extended Data Fig. 4: Global ribosome footprinting analysis of hem1 without ETI activation.
From: Plant HEM1 specifies a condensation domain to control immune gene translation
a, TE of the LUC translational reporter. Ribo-seq coverage on the LUC CDS region was normalized to RNA-seq as 1 for WT and hem1. TE was increased by 19% in hem1. b, Transcriptome changes (RSfc) of hem1 compared to WT. Blue dots, transcriptionally downregulated genes (RSdn; for example, genes in immune response); Pink dots, transcriptionally upregulated genes (RSup; for example, genes in cytoskeleton). P value, Wald test. c, d, GO analysis of RSup (c) and RSdn (d) genes; e, Translation efficiency changes (TEfc) of hem1 compared to WT. Blue dots, translationally downregulated genes (TEdn; for example, genes in immune response); Pink dots, translationally upregulated genes (TEup; for example, genes in cell cycle). f, g, GO analysis of TEup (c) and TEdn (d) genes. P value (c, d, f, g), Hyper Geometric test.
