Extended Data Fig. 2: GA signalling is broadly present during secondary growth.
From: Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
a, Expression of pRGA:GFP-RGA in 17-days old mock treated root. b, RGA expression is increased after 3-days on PAC treatment in 14-days old plants. The numbers ‘−2’, ‘−1’, ‘1’ and ‘2’ indicate the position of the cells relative to the most recent cell division, with negative values towards the xylem and positive towards the phloem. White arrowheads point to the most recent cell division. c, pRGA:GFP-RGA signal intensity quantifications from mock and PAC treated roots. d-f, Whole cross-sections of 17-day mock treated pRGA:GFP-RGA (d); pRGA:GFP-RGA after 3-day PAC treatment in 14-days old plants (e) and disappeared pRGA:GFP-RGA expression after 24 h GA treatment in 14-days old plants (f). Scale bars are 20 µm (a, b, d, e, f). Two-way ANOVA with Tukey’s post hoc test in c. The boxes in the box and whisker plots show the median and interquartile range, and the whiskers show the total range. Individual data points are plotted as purple dots. Boxes not sharing a lower-case letter in the plots indicate a significant difference, P < 0.05. ‘x’ = xylem, ‘p’ = phloem.
