Extended Data Fig. 2: Data collection and image processing.
From: Structural insights into a unique PSI–LHCI–LHCII–Lhcb9 supercomplex from moss Physcomitrium patens

a, b, Representative images of negative staining micrograph (a) and cryo-EM micrograph (b) of the PSI-LHCI-LHCII-Lhcb9 supercomplex from P. patens. The negative staining image was taken more than five times, and all resulted in similar particles. The cryo-EM micrograph was taken for 4,000 images, which were used in the subsequent structural analysis. c, Flow chart of the cryo-EM data processing for the Pp PSI-LHCI-LHCII-Lhcb9 supercomplex. d, Gold standard FSC curves of the final 3D reconstruction of the Pp PSI-LHCI-LHCII-Lhcb9 supercomplex. e, Local resolution distributions of the Pp PSI-LHCI-LHCII-Lhcb9 generated with cryoSPARC.