Extended Data Fig. 6: Basal SLY1s exhibit broader DELLA affinities.
From: Evolution of a plant growth-regulatory protein interaction specificity
a, Yeast-2-hybrid analysis of interactions between SLY1 and DELLA orthologues from various species (as indicated). b, Yeast-3-hybrid analysis of interactions between SLY, GID1 and DELLA orthologues from various species (as indicated). c, in vitro analysis of interactions between E.coli-expressed His-tagged AtGAI and MBP-tagged SLY1/GID2s. Anti-His serves as control and confirms that similar amounts of AtGAI protein were used to ‘pull down’ a SLY1/GID2 protein in each immunoprecipitation (IP) reaction, whilst anti-MBP shows how much SLY1/GID2 (quantified against anti-His, with MpSLY1 arbitrarily set at 1.00) was pulled down. d, in vitro analysis of interactions between E.coli-expressed His-tagged AtGAI and MBP-tagged SLY1/GID2s in the presence and absence of GST-AtGID1C and GA3. Anti-His serves as control and confirms that similar amounts of AtGAI protein were used to ‘pull down’ a SLY1/GID2 protein in each immunoprecipitation (IP) reaction, whilst anti-MBP shows how much SLY1/GID2 (quantified against anti-His, with SLY1/GID2 in the absence of GST-GID1C and GA3 arbitrarily set at 1.00) was pulled down. e, Mean plant heights, red dots indicate individual heights (n = 10), error bars indicate standard deviation, different letters (a and b) indicate significant difference (one-way ANOVA with Tukey’s test).
