Extended Data Fig. 8: SLY1 reversion routes are evolutionarily constrained.
From: Evolution of a plant growth-regulatory protein interaction specificity
a, Interactions of AtSLY1 and Atsly1 variants or MpSLY1 and Mpsly1 variants with GAI. b, Interactions of MpSLY1 and Mpsly1 variants with MpDELLA. c, Mean yeast-2-hybrid interaction strengths, Mpsly1 variants as in c, note gradient of increasing interaction strength ranging from Mpsly1Y108H to MpSLY1. ND = not detected; red dots indicate individual values (n = 3), error bars indicate standard deviation, different letters (a-d) indicate significant difference (one-way ANOVA with Tukey’s test). d, Interactions of MpSLY1 and AtSLY1 with 10 representative Arabidopsis GRAS family proteins. e, Interactions of AtSLY1 and Atsly1 variants with AtSCR. f, Interactions of AtSLY1 and Atsly1 variants with AtSCL4. g, Interactions of AtSLY1 and Atsly1 variants with GAI. h, Example mutant screen plates, mSmSLY1 (left), mAtSLY1 (centre), mOsGID2 (right). Positive candidates (blue colonies) are indicated with arrows.
