Fig. 4: Basal SLY1s exhibit broader DELLA affinities.
From: Evolution of a plant growth-regulatory protein interaction specificity
a, Phylogenetic tree showing SLY1/GID2 orthologues from M. polymorpha (MpSLY1), S. moellendorfii (SmSLY1), A. thaliana (AtSLY1) and rice (O. sativa; OsGID2) with MpSLY1L (MpSLY1-like) outgroup. The tree was constructed in MEGA11 using the maximum-likelihood method and the JTT matrix-based model. The percentage of trees in which the associated taxa clustered together (bootstrap value) is shown next to the branches (out of 500 bootstrap replicates). The tree is drawn to scale, with branch lengths measured as the number of substitutions per site. For a more comprehensive phylogeny, see Extended Data Fig. 5. b, Interactions between SLY1 and DELLA orthologues. c, Mean (±s.d.) yeast 2-hybrid SLY1 orthologue–GAI interaction strengths; note gradient of increasing interaction strength ranging from OsGID2 to MpSLY1. Red dots indicate individual values (n = 3), different letters (a–i) indicate significant differences (one-way ANOVA with Tukey’s test). d, Yeast 3-hybrid determination of relative affinities of SLY1 orthologues for GAI A and A’ forms.
