Extended Data Fig. 9: TRM1A can form complexes with pre-tRNAs containing a G26 or a A26.
From: A tRNA-modifying enzyme facilitates RNase P activity in Arabidopsis nuclei
Size exclusion chromatograms of recombinant AtTRM1A in presence of Sc-tRNAPhe containing a G26, At-tRNAmtCys containing a A26 and a fragment of At-COX1 mRNA. Red traces correspond to RNA alone. Green traces correspond to RNA and AtTRM1A mixed at a molar ratio of 1:2, blues traces correspond to RNA and AtTRM1A mixed at a molar ratio of 1:10, orange trace to TRM1A alone and purple trace to molecular weight markers. M1 is thyroglobulin (670 kDa), M2 is g-globulin (158 kDa), M3 is ovalbumin (44 kDa), M4 is myoglobin (17 kDa) and M5 is vitamin B12 (1,35 kDa). Grey arrows represent peaks corresponding to AtTRM1A / pre-tRNA complexes. x-axis shows the size exclusion chromatography elution time while the y-axis shows the OD measured at 260 nm for RNA containing samples or 280 nm for protein samples.
