Extended Data Fig. 1: Identification of double knock-out plants for PRORP2 and PRORP3 complemented by the insertion of a cDNA encoding PRORP2 fused with an HA tag.
From: A tRNA-modifying enzyme facilitates RNase P activity in Arabidopsis nuclei
a Gene models for AtPRORP2, AtPRORP3 and PRORP2-HA. Colour arrows and their names indicate primers used in b. T-DNA insertions, determined by Gutmann et al.14 are indicated by triangles. Expected sizes of the PCR products are indicated by the colour boxes under the gene models. b PCR products analysed on 1 % agarose gels. Colours in each image refer to the products indicated in gene models shown in a. WT plants are Columbia (Col-0) ecotype. PRORP2-HA was amplified from a cDNA representing the coding sequence without introns, which explains the difference in size in the PRORP2 gene PCR between WT plants and PRORP2-HA plants. These genotyping experiments were repeated three times.
