Extended Data Fig. 2: Analysis by western blot using anti-HA antibodies of fractions obtained during co-immunoprecipitation (IP) experiments.
From: A tRNA-modifying enzyme facilitates RNase P activity in Arabidopsis nuclei
Analysis by western blot using anti-HA antibodies of fractions obtained during co-immunoprecipitation (IP) experiments using a flower buds extract from an Arabidopsis stable plant line expressing a PRORP2-HA fusion protein (HA) and control wild-type plants (WT). IP were performed with anti-HA beads. Lysate, Flow through (FT), Wash (W) and elution (EL) samples were run on a 8 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane. M indicate molecular weight markers shown in kDa. The respective positions of the IgG heavy and light chains are indicated by grey arrows. A red arrow indicates the band corresponding to AtPRORP2-HA. This experiment was repeated three times.
