Extended Data Fig. 4: TRM1A and TRM1B are both localized to the nucleus.
From: A tRNA-modifying enzyme facilitates RNase P activity in Arabidopsis nuclei

Arabidopsis protoplasts were isolated, transfected with constructs expressing TRM1A-CFP and YFP-TRM1B and visualized by confocal microscopy. CFP signal is shown in cyan and YFP signal in yellow. DAPI signal in blue is used as a control to identify nuclei, that is nucleoplasm and the autofluorescence of chlorophyll in purple shows chloroplasts. Scale bars represent 10 mm. These microscopy observations were repeated three times.