Extended Data Fig. 8: AtPAT1 expression at inflorescence stem graft junctions and incised stems.
a, Schematic diagram showing the regions from the inflorescence graft junction for RNA extraction. 0.5 cm stem tissues collected from the scion and rootstock in grafted plants. A similar size and shape tissue was collected from intact plants as controls. b, c, Relative gene expression of AtANAC096 and AtPAT1 was analyzed by qRT-PCR in grafted plants at 0, 1, 3, 7 days after grafting (DAG). The mean (± SD) is shown from 3–6 biological replicates, 3 plants were pooled together per time point per treatment. d, Histochemical analysis of pPAT1:NLS–GFP/GUS at 3 or 7 DAG or days after incision (DAI). Left or middle panel is a longitudinal section through the graft junction (junctions indicated by a white rectangle). Right panel is a longitudinal section from an incised stem (wounding site is indicated by a white dashed circle). 2 biological replicates with 4 plants per treatment. Scales bars, 1 mm. e, pPAT1:NLS–GFP/GUS is activated at the grafted top and bottom 24 hours after grafting (n = 10), intact plant (n = 9) served as a control. Scale bars, 100 µm. 2 biological replicates.
