Fig. 2: Micrografting enables inter-genus grafting.
a, Phylogenetic tree showing the intergeneric relationships of Pinaceae based on published data34. b, Junction anatomy of homografted or heterografted Pinus contorta and Picea abies 4 or 6 months after grafting (MAG). Scale bars, 100 µm for images at 4 MAG, 1 mm for image at 6 MAG. n = 3 plants per combination per replicate, 2 biological replicates. c,e, Representative images of homografted or heterografted Pinus sylvestris, Pinus contorta and Picea abies 2.5 years after grafting. Intact (non-grafted) Pinus sylvestris and Pinus contorta were used as controls. Pinus sylvestris was used as scions in (c), and Pinus contorta was used as scions in (e). Scale bars, 10 cm. Inserts show the graft junctions. Scale bars, 1 cm. d,f, Height of 2.5-year-old intact and grafted Pinus sylvestris, Pinus contorta and Picea abies (mean ± s.d., n = 11–34 plants per combination). Pinus sylvestris was used as scions in (d), and Pinus contorta was used as scions in (f). Different letters above the bars represent P < 0.05. One-way ANOVA, with Tukey’s post hoc test. P values are shown in source data.
