Fig. 4: Picea abies grafting activates auxin-responsive and cell-wall-related gene expression.
a, Schematic diagram showing the regions collected for the hormone transcriptomes in Picea abies. About 50 mM auxin (IAA), 135 µM cytokinin (BAP) or auxin plus cytokinin (IAA + BAP) was applied, and tissues were collected 0 day, 5 days, 10 days and 15 days after treatment. b, The distribution of DEGs responding to hormone treatments. c,d, Venn diagrams showing the auxin-responsive genes (c) and cytokinin-responsive genes (d) differentially expressed by all three time points in the various treatments. e,f, An overlap analysis presented as a ratio of 1.0 for genes differentially expressed in response to hormones (auxin (e) and cytokinin (f)) and genes differentially expressed during Picea abies grafting (Extended Data Fig. 3c). Asterisks indicate statistically significant overlap between hormone response and graft healing. Significance was calculated by a Fisher test (one sided) with FDR adjustment. *P < 0.05. P values are shown in source data. g,h, Heat map showing the fold changes of putative laccase genes (g) and putative pectin methyl esterases (PMEs) (h) in graft healing and auxin datasets.
