Fig. 5: PAT1-related genes promote graft healing. | Nature Plants

Fig. 5: PAT1-related genes promote graft healing.

From: A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing

Fig. 5

a, Heat map of positively regulated transcriptional factors during graft healing. b, Regulatory connections of the top five core transcriptional factors based on a gene regulation network of transcription factors activated by grafting. The size and colour of nodes indicate the number of edge connections. The edge colour indicates the value of correlation. c, PaPAT1-like and AtPAT1 expression during graft formation in Picea abies and Arabidopsis. The mean (±s.d.) from two to three biological replicates is shown. d, Callus formation from cut Arabidopsis petioles overexpressing PAT1 (AtPAT1OE) (n = 40) or mutants for pat1scl5 (n = 41) or pat1scl5scl21 (n = 35). Scale bars, 100 µm. Two biological replicates. e, Callus area in wounded petiole explants in Col-0 (n = 28), AtPAT1OE (n = 40), pat1scl5 (n = 41) or pat1scl5scl21 (n = 35). Two biological replicates. f, Callus area in wounded petiole explants in Col-0 (n = 33), pat1 (n = 52), scl5 (n = 46), scl21 (n = 46) and scl5scl21 (n = 31). Three biological replicates. In panels e and f, significance was calculated using Wilcoxon’s test (two-sided) with FDR adjustment, compared with Col-0. Dots indicate individual samples, *P < 0.05, **** P < 0.0001; see source data for P values. g, Images and quantifications of callus formation from cut petioles of Col-0 (n = 10) and PaPAT1-like overexpression (n = 11). Scale bars, 100 µm. Dots indicate individual samples, two-sided Student’s t-test; ** P < 0.01. Two biological replicates. See source data for P values. h,i, Attachment (h) and phloem reconnection (i) rates of homografted Col-0, AtPAT1OE, pat1, scl5, scl21, pat1scl5, scl5scl21, pat1scl21 and pat1scl5scl21. j, Graft attachment rates of Col-0 and PaPAT1-like transformed in pat1scl5 and scl5scl21. In panels h–j, the mean (±s.d.) from 3 to 6 biological replicates with 10–17 plants per time point per experiment is shown. One-way ANOVA, with Tukey’s post hoc test. Different letters above the bars represent P < 0.05. P values are shown in source data.

Source data

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