Extended Data Fig. 3: Differentially expressed genes during Picea abies grafting.
a, b, Total differentially or non-differentially expressed genes in grafted Picea abies scions, rootstocks or intact tissues. Differential expression was determined by comparing to intact samples at the same time point (a) or by comparing to the day zero intact sample (b). c, d, Total number of upregulated and downregulated genes in scion, rootstock or intact Picea abies tissues. Differential expression was determined by comparing to intact samples at the same time point (c) or by comparing to the day zero intact sample (d). a-d, 3 biological replicates per tissue per treatment. e-h, Clustering analysis of transcriptional dynamics during graft healing. Lines indicate the average of differentially expressed genes in scion or rootstock and the expression profiles for selected Picea abies genes plotted over grafting. Dots indicate days after grafting (DAG). The number in the brackets represents the number of genes in the cluster. Cluster number is indicated and the mean (± SD) from 3 biological replicates per time points per tissue is shown. Arabidopsis homolog expression data are plotted and taken from published transcriptome data23 with a mean (± SEM) from 2 biological replicates per tissue per time point is shown.
