Extended Data Fig. 5: ADA2 protein is accumulated during the dark period.
(a) Nuclear proteins were extracted from rice seedlings (14 d after germination), grown on a 12-h diurnal light/dark cycle sampled at 6-h intervals from ZT12 to ZT6 for 2 days or with continuous darkness as indicated, followed by immunoblotting with anti-ADA2 and anti-H3 antibodies. (b) Endogenous ADA2 was immunoprecipitated from the nuclear proteins prepared as in (a) with anti-ADA2 antibody, followed by immunoblotting with anti-acetyl-lysine (anti-Lys-Ac) antibody to detect ADA2 acetylation. (c) The other replicate of Fig. 3A. (d) ADA2 transcript levels in rice seedlings indicated in (a). Transcript levels (n = 3, mean values from 3 measurements are presented) were measured by reverse transcription quantitative polymerase chain reaction (qPCR) and normalized using Actin as the reference gene.
