Extended Data Fig. 4: SDS-PAGE and immunoblotting analysis of PsaA and PsaF.
From: Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint
a, PsaA and PsaF protein detection in purified pre-PSI and mature PSI preparations. Left, Coomassie stained SDS-PAGE. Right, corresponding immunoblots for PsaA and PsaF. b, PsaA and PsaF protein detection in thylakoid preparations from etiolated greening (10 and 13 hours of light) and normal green seedlings. Left, SDS-PAGE of the total protein isolated from thylakoid membranes. Right, Immunoblots showing the protein expression levels of PsaA and PsaF. The anti-PsaF anybody has weak affinity, and longer exposure results in a secondary band with lower molecular weight. The experiments were repeated independently with similar results three times.
