Extended Data Fig. 3: Loss-of-function mutations of GSE3 lead to small grains.
From: Modulation of histone acetylation enables fully mechanized hybrid rice breeding
a, The target site in GSE3. Red letters represent the target sequence and blue letters represent the PAM sequence. The knockout line gse3-cri1 had a single base T insertion that caused a frame-shift, and gse3-cri2 had 30 bp base pair deletion. b, The knockout line gse3-cri1 was validated to have a single base T insertion in the second exon of GSE3 by sanger sequencing. c, The knockout line gse3-cri2 was validated to have the 30 bp deletion in the second exon of GSE3 by sanger sequencing. d, Mature rice grains of ZH11, gse3-cri1 and gse3-cri2. e-h, The grain length (e), grain width (f), grain thickness (g) and 1,000 grain weight (h) of ZH11, gse3-cri1 and gse3-cri2. n = 50 (e-f), n = 40 (g), and n = 3 (h). i-j, The tiller number (i) and grain number per plant (j) of ZH11, gse3-cri1 and gse3-cri2. n = 12 (i-j). Values in (e-j) are given as mean ± SD. **P < 0.01 compared with parental line (ZH11) by Student’s t-test. Bars: 3 mm (d).
