Extended Data Fig. 9: In vivo RSS of pri-miRNAs can better explain the first cleavage sites than in silico predicted structures.
From: Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis
(a) Barchart shows around 5% additional BTL-typed pri-miRNAs have internal loops/bulges that are 9-11 nt and 15-17 nt away from the first cleavage sites obtained in DRS vs RPS. (b) Barchart shows around 13% additional LTB-typed pri-miRNAs have internal loops/bulges that are 9-11 nt and 15-17 nt away from the first cleavage sites obtained in DRS vs RPS. (c) Venn diagram shows that both BTL- and LTB-typed pri-miRNAs concurrently present internal loops/bulges that are ~9-11 nt and ~15-17 nt away from the first cutting sites. RPS: RNAfold Predicted Structures. DRS: DMS Reactivity based Structures.
