Extended Data Fig. 5: Effects of Everlastin1 and Everlastin2 on petal senescence and gene expression in EPH1-knockout plants.

a, Time-course of visible senescence of the petal segments in EPH1-knockout (EPH1-KO) plants. Petal segments were floated adaxial side up in the DMSO control, Everlastin1, or Everlastin2 solution at a final concentration of 5 µM. Petal segments were prepared from five different flowers per treatment (Petri dish) and photographs of representative sets from at least three independent experiments are shown. b, Venn diagrams showing the overlap of the up-regulated (log₂ fold change >1) and down-regulated (log₂ fold change <−1) genes between EPH1-KO and Everlastin1 or Everlastin2 treatment compared to DMSO-treated WT control at t₈. c, Volcano plot showing differentially expressed genes between EPH1-KO and Everlastin1-treated petal segments at t₈. Red and blue plots indicate up-regulated (log₂ fold change >1 and p-value < 0.05) and down-regulated (log₂ fold change <−1 and p-value < 0.05) genes, respectively. p-value, Benjamini-Hochberg adjusted p-value. RNA-seq analysis was performed using three biological replicates per condition (b, c).