Extended Data Fig. 3: Effects of Everlastin1 and Everlastin2 on the DNA interaction of NAC TFs other than EPH1. | Nature Plants

Extended Data Fig. 3: Effects of Everlastin1 and Everlastin2 on the DNA interaction of NAC TFs other than EPH1.

From: A chemical approach to extend flower longevity of Japanese morning glory via inhibition of master senescence regulator EPHEMERAL1

Extended Data Fig. 3

a, Alignment of EPH1 (GenBank Accession No. AB849126), Arabidopsis ORE1 (NM_123323), and Lilium LhNAP (LC807006) polypeptide sequences. Identical amino acids are shown in reverse type. The overline indicates the conserved DNA-binding domain. b, Phylogenic tree of EPH1, Arabidopsis ORE1 and Lilium LhNAP proteins. Sequence alignment with ClustalW2 and phylogenic tree analysis with the neighbor-joining method were performed using Geneious Prime software (Biomatters, Auckland, New Zealand). c, Interaction assay for NAC TFs and DNA. Interactions between DNA and full-length ORE1 or LhNAP in the presence of Everlastin1 or Everlastin2 (5 µM each) were analyzed. DNA–protein interactions were analyzed using the AlphaScreen (AS) technology. Values are means ± s.e.m. of n = 4 independent experiments. Asterisks indicate significant differences. ns, not significant; **** P < 0.0001; one-way ANOVA with Dunnett’s test compared with DMSO.

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