Extended Data Fig. 4: Effects of Everlastin1 and Everlastin2 on petal senescence.

a, Petal segments treated with Everlastin1 or Everlastin2. Petal segments of I. nil were floated adaxial side up in DMSO control or Everlastin1 or Everlastin2 solutions at a final concentration of 5 µM. The pictures show petal segments at 24 h after flower opening (t24). Petal segments in the DMSO control showed water-soaking symptoms. Petal segments were prepared from five different flowers per treatment (Petri dish) and photographs of representative sets from at least three independent experiments are shown (a). b, Gel image of DNA degradation in petal segments treated with Everlastin1 or Everlastin2. A representative photograph from three biological replicates is shown. c, Cut flowers of I. nil treated with Everlastin2. Whole cut flowers were floated in Everlastin2 solution at a final concentration of 5 µM. The picture shows flowers at t24. A representative photograph from six biological replicates is shown.