Fig. 3: Validation of cell type annotation using RNA in situ hybridization.
From: Establishment of single-cell transcriptional states during seed germination
a,b, Expression domains of independent marker transcripts of cluster 9 (a) and cluster 14 (b) confirmed the physical location of the cells in these clusters. UMAP dimensional reduction graph visualized the expression of marker transcript at 48 h. The hybridization signals were detected at 48 but not 24 h, also confirming the temporal detection of these clusters in scRNA-seq data. For each time point, the results of hybridization with antisense probes (that is, the test) and sense probes (negative controls) are shown. Representative images are shown (more than ten embryos were examined). Scale bars, 200 μm.
