Extended Data Fig. 3: Cryo-EM analysis of AtABCG16inward-JA.
From: Cryo-EM structure and molecular mechanism of the jasmonic acid transporter ABCG16
a, Gel filtration profile of a Superdex-200 column and Coomassie-blue-stained SDS-PAGE analysis of AtABCG16 in buffer containing 0.05% digitonin. Independent experiments have been repeated at least three times with similar results. b, Representative micrograph. c, 2D class averages. d, cryo-EM data analysis pipeline. e, Local resolution estimation and gold-standard Fourier shell correlation (FSC) curves. The color represents the local resolution in Å. f, cryo-EM density of representative segments superimposed with the atomic model (map contour level = 6σ) Source data.
