Extended Data Fig. 4: Cryo-EM analysis of AtABCG16outward-open.
From: Cryo-EM structure and molecular mechanism of the jasmonic acid transporter ABCG16
a, Gel filtration profile of a Superose 6 column and Coomassie-blue-stained SDS-PAGE analysis of AtABCG16 in buffer containing 0.01% LMNG + 0.001% CHS + 0.0033% GDN. Independent experiments have been repeated at least three times with similar results. b, Representative micrograph. c, 2D class averages. d, cryo-EM data analysis pipeline. e, Local resolution estimation and gold-standard Fourier shell correlation (FSC) curves. The color represents the local resolution in Å. f, cryo-EM density of representative segments superimposed with the atomic model (map contour level = 6σ).
