Extended Data Fig. 8: Matrix-traversing membrane-localized proteins CYN7 and CAH3 are mis-localized in mith1 and saga1 mutants.
From: SAGA1 and MITH1 produce matrix-traversing membranes in the CO2-fixing pyrenoid
a-d, Images of Venus and chlorophyll channels in a wild-type control not expressing Venus (a), a wild-type strain expressing CYN7-Venus (b), mith1 expressing CYN7-Venus, and saga1 expressing CYN7-Venus (d). Images are presented at the same scale. Arrowheads point to the wild-type matrix-traversing membrane tubules and mith1 pyrenoid-associated membranes labeled by CYN7-Venus. e, Additional images of CYN7-Venus expression in live cells of the wild-type, mith1, and saga1 strains. Arrowheads point to bright CYN7-Venus signal associated with the pyrenoid. f, Immunofluorescence with an anti-CAH3 antibody was used to visualize localization of endogenous CAH3. Arrowheads point to bright CAH3 signal associated with the pyrenoid. In mith1 mutants, CAH3 localized to small puncta and streaks at the periphery of the pyrenoid rather than to the star-like traversing network seen in wild-type cells. The cah3 mutant was used as a negative control. Images are presented at the same scale as (e).
