Extended Data Fig. 2: MITH1-mCerulean and SAGA1-mCherry were heterologously expressed in an Arabidopsis line containing CrRBCS1 and EPYC1-GFP. | Nature Plants

Extended Data Fig. 2: MITH1-mCerulean and SAGA1-mCherry were heterologously expressed in an Arabidopsis line containing CrRBCS1 and EPYC1-GFP.

From: SAGA1 and MITH1 produce matrix-traversing membranes in the CO2-fixing pyrenoid

Extended Data Fig. 2

Western blots were performed on soluble protein extracts run through a denaturing SDS-PAGE gel to probe for a, proteins expressed in the Arabidopsis CrRBCS2;EPYC1-GFP;MITH1-mCerulean;SAGA1-mCherry line (lane 1) and CrRBCS2;EPYC1-GFP line (lane 2) as compared to the native proteins from Chlamydomonas (lane 3) and b, proteins expressed in the CrRBCS2;EPYC1-GFP line (lane 1) compared to CrRBCS2;EPYC1-GFP;MITH1-mCerulean plants (lanes 2-5). The anti-GFP antibody was used to detect mCerulean. In (a), Actin is used as a loading control for the Arabidopsis samples. In (b), a Ponceau stain is used to detect total protein as a loading control. Predicted molecular weights are as follows: SAGA1: 168 kDa, SAGA1-mCherry: 195 kDa, MITH1: 139 kDa, MITH1-mCerulean: 171 kDa, EPYC1: 32 kDa, EPYC1-GFP: 59 kDa.

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