Extended Data Fig. 4: In Arabidopsis, SAGA1-mCherry and MITH1-mCerulean are both necessary to generate matrix-traversing membranes.
From: SAGA1 and MITH1 produce matrix-traversing membranes in the CO2-fixing pyrenoid
a, TEM images of the chloroplasts of the CrRBCS2 line and lines expressing EPYC1-GFP, SAGA1-mCherry, and MITH1-mCerulean. b, Example images of the CrRBCS2;EPYC1-GFP;SAGA1-mCherry;MITH1-mCerulean line where MITH1 is expressed under either the stronger CaMV35S promoter or a weaker promoter, AtRbcS3B. c, Additional images of heterologous protein localizations in the Arabidopsis lines are shown. d, Z-stack depicting the overlay of channels for the CrRBCS2;EPYC1-GFP;SAGA1-mCherry;MITH1-mCerulean pyrenoid shown in Fig. 2e. e, Example TEM of the CrRBCS2;EPYC1-GFP;SAGA1-mCherry;MITH1-mCerulean highlighting the apparent presence of a double membrane. Gray values were measured across the blue line and were used to produce a graph plotting the gray value across the line. f, Heatmap depicting gray values measured for 8 traversing membranes in different cells. Within each panel, all images are shown at the same scale.
