Extended Data Fig. 6: Functional characterization of SOH1-LIKEs from Oryza sativa and Physcomitrium patens.
a and b, PpSOH1-LIKE1 and OsSOH1-LIKE physically interact with AtCER3 in N. benthamiana cell by luciferase complementation imaging (LCI) assay, N-terminus of luciferase fused PpSOH1-LIKE1 and OsSOH1-LIKE, AtCER3 fused C-terminus luciferase co-transformed N. benthamiana cells exhibit luciferase activity, BlpR (phosphinothricin acetyltransferase) and KanR (aminoglycoside phosphotransferase) were used as negative control. These results were independently replicated at least twice with similar outcomes. c, C26, C28 and C30 alcohols generated by the yeast cells co-expressing PpSOH1-LIKE1 or OsSOH1-LIKE with AtCER3. Gas chromatography-flame ionization detection (GC-FID) of the alcohol fractions after separation of total lipid from different yeast strains using thin layer chromatography (TLC). These results were independently replicated three times with similar outcomes. d and e, primary alcohol coverage in the leaves of different PpSOH1-LIKE1 (d) and OsSOH1-LIKE (e) overexpression lines, respectively. Wax coverage is expressed as microgram per square decimeter of leaf surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 4 of biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s two-tailed t-test). Exact P values are listed in the source data. f and g, alkane coverage in the leaves of different PpSOH1-LIKE1 (f) and OsSOH1-LIKE (g) overexpression lines. Wax coverage is expressed as microgram per square decimeter of leaf surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 4 of biological replicates. *P < 0.05 (Student’s two-tailed t-test). Exact P values are listed in the source data.
